产品[
Introductory EcoPro™ T7 System
]资料
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产品名称:
Introductory EcoPro™ T7 System
产品型号:
70888
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
Introductory EcoPro™ T7 SystemIn the EcoPro™ T7 System, the extract is supplemented with T7 RNA polymerase, such that protein synthesis is directed from T7 promoters in addition to E. coli
Introductory EcoPro™ T7 System
的详细介绍
Introductory EcoPro™ T7 System
| 产品信息 |
|---|
| Avoid freeze/thaw | Yes |
| Components | | • | 350 µl | EcoPro T7 Extract
| | • | 100 µl | 5 mM Methionine
| | • | 1.5 ml | Nuclease-free Water
| | • | 5 µg | EcoPro Control DNA |
|
| Store and ship information |
|---|
| Storage | ≤ -70°C |
| Ship | Dry Ice Only
Standard Handling |
In the EcoPro™ T7 System, the extract is supplemented with T7 RNA polymerase, such that protein synthesis is directed from T7 promoters in addition to E. coli promoters. Optimal translation is achieved using templates that carry a strong prokaryotic ribosome binding site (RBS; e.g. AAGGAG) 6–11 base pairs upstream from the ATG initiation codon. The EcoPro T7 extract contains optimal levels of all components needed for template-dependent transcription and translation, reducing the time and expense of reaction assembly. The EcoPro T7 System can be easily integrated with a variety of non-isotopic detection methods including the FRETWorks™ S•Tag™ Assay.