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ProteoEnrich™ CAT-X Kit
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产品名称:
ProteoEnrich™ CAT-X Kit
产品型号:
71532
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
ProteoEnrich™ CAT-X Kit Highly specific, reproducible, convenient methods to reduce proteome complexity
ProteoEnrich™ CAT-X Kit
的详细介绍
ProteoEnrich™ CAT-X Kit
| 产品信息 |
|---|
| Components | | • | 2 each | CAT-X Cartridge
| | • | 50 ml | 10X CAT-X Buffer
| | • | 90 ml | 5 M NaCl
| | • | 1 pkg | Luer Lock Adaptors |
|
| Store and ship information |
|---|
| Storage | +2°C to +8°C |
| Ship | Blue or Dry Ice
Standard Handling |
The ProteoEnrich™ CAT-X Kit provides a convenient and reproducible method to fractionate the proteome of a given biological sample under non-denaturing conditions prior to downstream analysis by one- and two-dimensional gel electrophoresis, liquid chromatography, protein arrays and functional tests such as activity assays and ELISA. The ProteoEnrich CAT-X Kit procedure fractionates proteins based on their binding to the strong cation exchange resin, Fractogel® EMD SO3. Nearly the complete proteome binds to the matrix under slightly acidic conditions, and partial proteomes can be eluted with a salt gradient. Excess salt should be removed prior to 2D gel electrophoresis. The kit contains two CAT-X cartridges with Luer Lock Adapters, and optimized buffers. The CAT-X cartridges can be used as syringe-tip filters or as liquid chromatography devices. Each cartridge binds up to 140 mg of a single protein or up to 5 mg of a complex protein mixture and can be reused at least 10 times.