产品[
novagen Overnight Express™ Autoinduction System 1自动诱导系统1
]资料
如果您对该产品感兴趣的话,可以
产品名称:
novagen Overnight Express™ Autoinduction System 1自动诱导系统1
产品型号:
71300
产品展商:
上海嘉适科学仪器有限公司
产品文档:
无相关文档
简单介绍
novagen Overnight Express™ Autoinduction System 1自动诱导系统1 在不含葡萄糖的培养基，入LB或TB 加入以上几种组分，用pET或其他IPTG诱导系统表达蛋白时侧可以实现自动诱导蛋白表达，不仅细胞生长密度高，而且可容目的蛋白产量也大大增加。
novagen Overnight Express™ Autoinduction System 1自动诱导系统1
的详细介绍
novagen Overnight Express™ Autoinduction System 1自动诱导系统1包含三种溶液，OnEx™溶液1是经过优化的提供碳源的混合物，用以眼睛调控非诱导的细胞生长和乳糖诱导的蛋白表达；OnEx™溶液2是缓冲也，用于在代谢产酸过程维持ph值以及提供合成蛋白所必需的氮。OnEx™溶液3提供对于细胞中生长至最大密度的的镁离子。在不含葡萄糖的培养基，入LB或TB 加入以上几种组分，用pET或其他IPTG诱导系统表达蛋白时侧可以实现自动诱导蛋白表达，不仅细胞生长密度高，而且可容目的蛋白产量也大大增加。
名称 | 品牌 | 规格 | 原始型号 | 价格 |
novagen Overnight Express™ Autoinduction System 1自动诱导系统1 | Novagen | 1 kit | 71300-3 | |
Novagen | 1 kit | 71300-4 | |
细菌细胞生长和蛋白表达
Medium | protein | OE12 | IPTG | OE1 | IPTG |
TB | β-gal | 20.7 | 9.7 | 675 | 225 |
TB | Musa | 20.0 | 8.5 | 690 | 240 |
TB | GST | 20.2 | 15.2 | 750 | 255 |
novagen Overnight Express™ Autoinduction System 1自动诱导系统1
1纯化的蛋白产量用BCA蛋白检测试剂盒监测，单位为μg/ml培养物
2单克隆菌接种到含有Overnight Express™系统1的2ml培养基中，用17x100mm的带有卡口瓶盖的圆底瓶30℃ 300rpm摇菌过夜（约16小时）
3单克隆菌接种到有0.5%葡萄糖的2ml培养基中，用17x100mm 用30℃ 300rpm摇菌至OD600=2.2时，加入IPTG之终浓度为1mm，继续孵育5小时，收集菌液。IPTG诱导的培养基中含溶液3，从而在于Overnight Express™系统1诱导表达比较时，消除了mgso4可能引起的差异。
With Overnight Express Autoinduction System 1, a period of cell growth is followed by spontaneous induction of protein expression—without monitoring cell density and without conventional induction with IPTG. The method is based on media components that are metabolized differentially to promote growth to high density and automatically induce protein expression from lac promoters.
The kit contains three components: OnEx™ Solution 1, OnEx Solution 2, and OnEx Solution 3. OnEx Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high cell density followed by high-level induction. OnEx Solution 2 is a concentrated buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis. OnEx Solution 3 provides magnesium for maximal cell density. Addition of these components to traditional glucose-free E. coli culture media, such as LB broth, TB, or animal-free Veggie™ media results in high cell densities, autoinduction of expression, and maximum soluble protein yields (Grabski 2003).
The Overnight Express Autoinduction System is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones. The Overnight Express Autoinduction System together with the Novagen RoboPop™ Solubility Screening Kit and RoboPop Purification Kits allow rapid optimization of the host-vector combinations, expression conditions, and purification parameters for high-throughput production of proteins for structural or functional analysis.
High-level protein expression without the need to monitor cell growth The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli without monitoring the culture or adding inducer during cell growth. These unique culture media are based on technology by F. William Studier at Brookhaven National Laboratory. The products include Overnight Express Instant TB Medium, System 1, and System 2. The granulated Instant TB Medium formulation combines Overnight Express System 1 and Terrific Broth (TB) into a rich culture medium, making autoinduction of protein expression even more convenient. The Overnight Express Autoinduction Systems feature high-level protein production in the pET and other IPTGinducible bacterial expression systems without the need to monitor cell growth. Cell mass and target protein yield are often increased severalfold as compared with conventional protocols using induction with IPTG.
Overnight Express Instant TB Medium
Overnight Express Instant TB Medium is a complete granulated culture medium for high-level protein production in the pET and other IPTG-inducible bacterial expression systems without the need to monitor cell growth. Cell mass and target protein yield are often increased several-fold as compared with conventional protocols using induction with IPTG. The method is based on media components that are metabolized differentially to promote growth to high density and automatically induce protein expression from lac promoters. The Overnight Express Instant TB Medium is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones.
Overnight Express Instant TB Medium is supplied in two formats. EasyPak is an aluminum foil pouch containing 60 g granulated medium sufficient for 1 L culture. Just add the EasyPak contents to 1 L water, supplement with 10 ml glycerol, and microwave for 2 minutes to sterilize. In addition, the Overnight Express Instant TB Medium is supplied in 1 kg bottles. The granules ensure rapid and uniform dissolution in water, and prevent clumping of the medium and inhalation of the airborne powder.
Overnight Express Autoinduction System 1
With Overnight Express™ Autoinduction System 1, a period of cell growth is followed by spontaneous induction of protein expression–without monitoring cell density and without conventional induction with IPTG. The method is based on media components that are metabolized differentially to promote growth to high density and automatically induce protein expression from lac promoters.
The kit contains three components, OnEx™ Solutions 1, 2, and 3. OnEx Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high cell density, followed by high-level induction. OnEx Solution 2 is a concentrated buffer and nitrogen-source blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis. OnEx Solution 3 provides magnesium for maximal cell density. Addition of these components to traditional glucose-free E. coli culture media, such as LB broth or TB media results in high cell densities, autoinduction of expression, and maximum soluble protein yields (Grabski, et al 2003).
The Overnight Express Autoinduction System is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones. The Overnight Express Autoinduction System together with the Novagen RoboPop™ Solubility Screening Kit and RoboPop Purification Kits allow rapid optimization of host-vector combinations, expression conditions, and purification parameters for high-throughput production of proteins for structural or functional analysis.
Overnight Express Autoinduction System 2
Overnight Express™ Autoinduction System 2 provides a complete, chemically defined medium for high-level protein expression with the pET System, and other IPTG-inducible expression systems, without the need to monitor cell growth. All system components have a known chemical composition, resulting in consistent product performance and the elimination of lot-to-lot variability. System 2 also features the capability to label proteins with selenomethionine (Se-Met) for downstream crystallization and x-ray diffraction studies.
The system includes six concentrated sterile solutions: OnEx™ solutions 1 through 6. OnEx Solutions 1, 2, and 3 are the same components included in the Overnight Express System 1 (see preceding page). OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the metal requirements of metal-containing target proteins, even at high expression levels. OnEx Solution 5 is a mixture of amino acids lacking methionine. OnEx Solution 6 is a separate methionine solution. Sufficient methionine (Met) is provided to support growth of the Met auxotroph B834 while providing the ability to reduce the level of unlabeled Met for selenomethionine incorporation by Met auxotrophs.
The Overnight Express Autoinduction System 2 is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility screening, and purification from multiple expression clones. The tedium of preparing a defined medium from dozens of components has been simplified with the ready-to-use sterile solutions included in the Overnight Express Autoinduction System 2.
Two Overnight Express™ Autoinduction Systems (System 1 and System 2) are available, both featuring high-level protein production in the pET and other IPTG-inducible bacterial expression systems without the need to monitor cell growth. Cell mass and target protein yield are often increased several-fold as compared with conventional protocols using induction with IPTG. Also available is Overnight Express Instant TB Medium.
| 产品信息 |
|---|
| Features and benefits | - No need to monitor cell growth or add IPTG
- High cell densities and protein expression levels
- Increased soluble protein yield
- Induction of numerous expression clones simultaneously
- Usable with any conventional glucose-free bacterial growth medium
- Ideal for pET Expression System or other IPTG-inducible bacterial systems
- Compatible with cultures grown in flasks, culture tubes, and deep-well plates
- Minimal sample handling
|
| Store and ship information |
|---|
|
| Storage | +15°C to +30°C |
| Ship | Ambient Temperature Only
Standard Handling |
novagen Overnight Express™ Autoinduction System 1自动诱导系统1novagen Overnight Express™ Autoinduction System 1自动诱导系统1
novagen Overnight Express™ Autoinduction System 1自动诱导系统1novagen Overnight Express™ Autoinduction System 1自动诱导系统1
novagen Overnight Express™ Autoinduction System 1自动诱导系统1