产品[
GE 脱盐柱 PD MiniTrap G-25
]资料
如果您对该产品感兴趣的话,可以
产品名称:
GE 脱盐柱 PD MiniTrap G-25
产品型号:
28-9180-07
产品展商:
上海嘉适科学仪器有限公司
产品文档:
无相关文档
简单介绍
GE 脱盐柱 PD MiniTrap G-25 可靠有效的清除蛋白和生物大分子(> 5000 Mr)
GE 脱盐柱 PD MiniTrap G-25
的详细介绍
名称 | 品牌 | 规格 | 原始型号 | 价格 |
GE 脱盐柱 PD MiniTrap G-25 | GE | 50 columns | 28-9180-07 | 现询 |
PD MiniTrap G-25
- Reliable and reproducible cleanup of proteins and other large biomolecules (> 5000 Mr).
- Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
- High desalting capacity.
- Flexible protocols with ability to use gravity flow or centrifugation.
- Sample preparation in the range of 0.1 to 0.5 ml for gravity and 0.2 to 0.5 ml with centrifugation.
PD MiniTrap G-25
Technical Information
PD MiniTrap G-25 is designed for convenient desalting and buffer exchange of biological samples. For increased flexibility, the product has two alternative application protocols, using either gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
PD MiniTrap G-25 is a prepacked column for cleanup of biomolecules with a molecular weight > 5000 in sample volumes up to 0.5 ml.
Removal of NaCl from BSA using the gravity protocol. Sample recovery was 95% and the desalting capacity was > 99%.
| TECHNICAL SPECIFICATIONS |
| Medium | Sephadex G-25 Medium |
| Separation mechanism | Gel filtration |
| Particle size range | 85 to 260 μm |
| Exclusion limit | Mr 5000 |
| Sample volume (gravity) | 0.1 to 0.5 ml |
| Sample volume (spin) | 0.2 to 0.5 ml |
| Column volume | 5 ml |
| Medium volume | 2.1 ml |
| Recovery* | 70% to 95% |
| Desalting capacity | > 90% |
| Chemical stability | All commonly used buffers |
| Working pH range | 2 to 13 |
| Storage | 4°C to 30°C |
| Column material | Polypropylene barrel and polyethylene frits |
| * Biomolecule dependent |
GE 脱盐柱 PD MiniTrap G-25