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重组溶菌酶 rLysozyme™ Solution
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产品名称:
重组溶菌酶 rLysozyme™ Solution
产品型号:
71110-3
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
重组溶菌酶 rLysozyme™ Solution ；溶液包含高纯度的，功能稳定的重组溶菌酶，用于裂解E. coli. 该酶催化细胞壁中的N-乙酰胞壁酰胺键水解，rLysozyme™ 特殊的大肠杆菌裂解活性(1700 KU/mg)比蛋清来源的溶菌酶高250倍。
重组溶菌酶 rLysozyme™ Solution
的详细介绍
名称 | 品牌 | 规格 | 原始型号 | 价格 |
重组溶菌酶 rLysozyme™ Solution | | 300K | 71110-3 | 现询 |
Novagen | 1200KU | 71110-3 | 现询 |
Novagen | 6000KU | 71110-3 | 现询 |
重组溶菌酶 rLysozyme™ Solution
rLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3-5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45-60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration of 30 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at -20°C.
| 产品信息 |
|---|
| Avoid freeze/thaw | Yes |
| Unit definition | One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A450 unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5. |
| Store and ship information |
|---|
|
| Storage | -20°C |
| Ship | Blue or Dry Ice
Standard Handling |