产品[
novagen酵母蛋白抽提试剂 植物蛋白抽提
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产品名称:
novagen酵母蛋白抽提试剂 植物蛋白抽提
产品型号:
71186 YeastBuster? Protein Extraction Reagent
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
novagen酵母蛋白抽提试剂 植物蛋白抽提:不需机械处理和酶法裂解直接从酵母蛋白和植物细胞中提取可溶蛋白。采用试剂处理,可以避免激烈的机械处理制造成的氧化和热度升高对目的蛋白的破环作用,这种试剂专利配方包括性质温和的去污剂,蛋白稳定剂,tris磷酸(THP)还原试剂(单独提供)
novagen酵母蛋白抽提试剂 植物蛋白抽提
的详细介绍
名称 | 品牌 | 规格 | 原始型号 | 价格 |
| Novagen | 100ml | 71186-3 | 现询 |
Novagen | 500ml | 71186-4 | 现询 |
novagen酵母蛋白抽提试剂 植物蛋白抽提
novagen酵母蛋白抽提试剂设计用于快速,高效而温和的抽提可溶活性蛋白。
采用试剂处理,可以避免激烈的机械处理制造成的氧化和热度升高对目的蛋白的破环作用,这种试剂专利配方包括性质温和的去污剂,蛋白稳定剂,tris磷酸(THP)还原试剂(单独提供),这种使用方便的试剂可以避免采用玻璃珠研磨细胞,超声破处理或压榨法等传统机械式操作或用13-葡聚糖酶裂解酶消化。盖试剂已在Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe酵母细胞及植物细胞中进行测试。
使用时先离心收集细胞,再将细胞用YeastBuster™重悬,短时孵育后,离心去掉细胞残片,粗粗提物即可用于后续实验。
这样处理得到的粗提物中蛋白量大,活性蛋白比例高,并且完全适用GST•Bind™ 和Ni-NTA His•Bind®固定化金属色谱(IMAC)纯化方法进行纯化。该试剂以100ml和500ml2种包装提供
novagen酵母蛋白抽提试剂 植物蛋白抽提
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.



novagen酵母蛋白抽提试剂 植物蛋白抽提
| Product information |
|---|
| Components | | • | 100 ml or 500 ml | YeastBuster™ Protein Extraction Reagent | | • | 1 ml or 5 ml | 100X THP Solution |
|
| Features and benefits | - Gentle, rapid, efficient extraction of proteins from yeast cells
- Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
- Higher yield of total and enzymatically active proteins
- Fully compatible with Ni-NTA His•Bind® and GST•Bind™ affinity purification methods
|
| Store and ship information |
|---|
|
| Storage | Multiple storage requirements |
| Ship | Blue or Dry Ice Multiple Toxicity Values, refer to MSDS |