产品[
昆虫细胞蛋白抽提试剂 Insect PopCulture® Reagent
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产品名称:
昆虫细胞蛋白抽提试剂 Insect PopCulture® Reagent
产品型号:
杆状病毒表达 从昆虫细胞培养物中抽提蛋白
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
昆虫细胞蛋白抽提试剂 Insect PopCulture® Reagent ：无需从培养基中分离细胞；纯化前无需对细胞粗提物进行处理；回收培养基中蛋白及抽提物细胞内蛋白，从而提高细胞产量；直接从培养体系粗提物中亲和纯化目的蛋白；与蛋白酶抑制剂兼容；适用于高通量蛋白表达水平检测和蛋白纯化；适用于传染和杆状病毒感染的昆虫细胞培养
昆虫细胞蛋白抽提试剂 Insect PopCulture® Reagent
的详细介绍
名称 | 品牌 | 规格 | 原始型号 | 价格 |
昆虫细胞蛋白抽提试剂
Insect PopCulture® Reagent | | 50ml | 71187-3 | 现询 |
Novagen | 250ml | 71187-4 | 现询 |
直接从昆虫细胞培养物中抽提蛋白，昆虫PopCulture®试剂是基于去污剂配方的细胞裂解试剂，可以不需离心收集细胞，直接从昆虫细胞培养物中抽提蛋白。该试剂经过优化，既可以回收释放到培养基中的蛋白，也可以抽提细胞内蛋白，从而提高抽提效率和目的蛋白的产量。改试剂与自己检测蛋白表达水平相兼容，也与Ni-NTA His•Bind®亲和纯化相兼容。昆虫PopCulture®试剂既可以用于悬浮生长的昆虫细胞，也可以用于在组织培养板上贴壁生长的昆虫细胞的蛋白抽提
昆虫细胞蛋白抽提试剂 Insect PopCulture® Reagent
特点：无需从培养基中分离细胞；纯化前无需对细胞粗提物进行处理；回收培养基中蛋白及抽提物细胞内蛋白，从而提高细胞产量；直接从培养体系粗提物中亲和纯化目的蛋白；与蛋白酶抑制剂兼容；适用于高通量蛋白表达水平检测和蛋白纯化；适用于传染和杆状病毒感染的昆虫细胞培养
Insect PopCulture® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind® affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.
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| Product information |
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| Features and benefits | - No need to separate cells from culture media
- No need to clarify cell extracts prior to purification
- Higher protein yield due to target protein recovery from both medium and cells
- Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
- Compatible with protease inhibitor cocktails
- Ideal for high-throughput, expression-level screening and protein purification
- Compatible with transfected and baculovirus-infected insect cell cultures
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| Store and ship information |
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| Storage | +15°C to +30°C |
| Ship | Ambient Temperature Only
Irritant |