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凝胶过滤预装柱 Superdex Peptide 10/300 GL
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产品名称:
凝胶过滤预装柱 Superdex Peptide 10/300 GL
产品型号:
17-5176-01
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
凝胶过滤预装柱 Superdex Peptide 10/300 GL： 肽及其它小分子的半制备和高性能分析纯化 球蛋白分离范围：100-7,000.
凝胶过滤预装柱 Superdex Peptide 10/300 GL
的详细介绍
Superdex Peptide, 75 and 200 prepacked in Tricorn columns offer unmatched high-performance gel filtration. 凝胶过滤预装柱 Superdex Peptide 10/300 GL
| TECHNICAL SPECIFICATIONS |
| Superdex™ Peptide |
| Fractionation range (Mr) | 100-7000 |
| Matrix | Spherical composite of cross-linked agarose and dextran |
| Average particle size | 13 µm |
| Chemical stability | Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning-in-place) |
| pH stability | 1-14 (working, long and short term) |
| Prepacked Superdex™ Peptide Columns* |
| Bed volume | |
| PC 3.2/30 | 2.4 ml |
| Tricorn 10/300 GL | 24 ml |
| Sample volume | |
| PC 3.2/30 | 2-25 µl |
| Tricorn 10/300 GL | 25-250 µl |
| Max. pressure | |
| PC 3.2/30 | 20 bar (290 psi, 2 MPa) |
| Tricorn 10/300 GL | 15 bar (220 psi, 1.5 MPa) |
| Max. flow rate (H2O at 25°C) |
| PC 3.2/30 | 150 µl/min |
| Tricorn 10/300 GL | 1.2 ml/min |
| Theoretical plates | > 30 000 m-1 |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Columns are not suitable for use with ÄKTAprime™ plus system. |
Separation of standard peptides on Superdex™ Peptide 10/300 GL.
The separation of SPI peptides C0070 illustrates the difference in resolution for small peptides.The relative pore volume of Superdex™ Peptide is 55.5% compared to 39.1% for TSK G2000 SW.
Superdex™ Peptide Columns
Technical Information
Reference
- Fägerstam, L. et al. Detection and characterisation of oligosaccharides in column effluents using surface plasmon resonance. Fourteenth International Symposium on the Separation and Analysis of Proteins, Peptides, and Polynucleotides, Heidelberg, Germany (1994).
凝胶过滤预装柱 Superdex Peptide 10/300 GL凝胶过滤预装柱 Superdex Peptide 10/300 GL