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GE凝胶过滤预装柱 SupeSuperdex 75 10/300 GL

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产品名称: GE凝胶过滤预装柱 SupeSuperdex 75 10/300 GL
产品型号: 17-5174-01
产品展商: 上海嘉适科学仪器有限公司
产品文档: 无相关文档


简单介绍
GE凝胶过滤预装柱 SupeSuperdex 75 10/300 GL :蛋白,DNA片段及其它小分子的半制备和分析纯化。球蛋白分离范围:10,000-600,000

GE凝胶过滤预装柱 SupeSuperdex 75 10/300 GL

的详细介绍
GE凝胶过滤预装柱 SupeSuperdex 75 10/300 GL

Technical Information


*Tricorn columns are design patented, see back of catalog for details.

 

TECHNICAL SPECIFICATIONS
Superdex™
Exclusion limit (Mr
Superdex™ 75 1 × 105 globular protein 
Superdex™ 200 1.3 × 106 globular protein 
Separation range (Mr
Superdex™ 75 3000-70 000 globular protein 
Superdex™ 200 10 000-600 000 globular protein 
Matrix  Spherical composite of cross-linked agarose and dextran 
Average particle size 13 µm 
Chemical stability Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning in place) 
pH stability 3-12 (working and long term), 1-14 (short term) 
Superdex™ 10/300 GL Columns (Tricorn)*
Bed dimensions 10 × 300 mm 
Recommended sample volume 25-250 µl 
Bed volume 24 ml 
Max. pressure  
Superdex™ 75 18 bar (261 psi, 1.8 MPa) 
Superdex™ 200 15 bar (217 psi, 1.5 MPa) 
Max. flow rate (H2O at 25°C) 
Superdex™ 75 1.5 ml/min 
Superdex™ 200 1.0 ml/min 
Theoretical plates > 30 000 m-1 
Superdex™ PC 3.2/30 Columns
Bed dimensions 3.2 × 300 mm 
Bed volume 2.4 ml 
Recommended sample volume 2-25 µl 
Max. pressure  
Superdex™ 75 24 bar (348 psi, 2.4 MPa) 
Superdex™ 200 12 bar (174 psi, 1.2 MPa) 
Max. flow rate (H2O at 25°C) 
Superdex™ 75 0.100 ml/min 
Superdex™ 200 0.100 ml/min 
Theoretical Plates > 30 000 m-1 
Storage 20% ethanol 
Storage temperature 4°C to 30°C 
* Columns are not suitable for use with ÄKTAprime™ plus system.

 

 

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Separation of standard proteins on Tricorn™ Superdex™ 200.

 

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Separation of monomer and dimer on Tricorn™ Superdex™ 75.
GE凝胶过滤预装柱 SupeSuperdex 75 10/300 GLGE凝胶过滤预装柱 SupeSuperdex 75 10/300 GL

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