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GE凝胶过滤预装住 HiLoad™ Superdex™ prep grade Columns
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产品名称:
GE凝胶过滤预装住 HiLoad™ Superdex™ prep grade Columns
产品型号:
GE Pharmacia 17-1069-01
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
GE凝胶过滤预装住 HiLoad™ Superdex™ prep grade Columns ：蛋白，DNA片段及其它小分子的快速制备纯化。
GE凝胶过滤预装住 HiLoad™ Superdex™ prep grade Columns
的详细介绍
GE凝胶过滤预装住 HiLoad™ Superdex™ prep grade Columns
- Exceptional resolution with short run times and good recovery
- Superdex 30 prep grade for polishing and formulation of peptides
- Superdex 75 prep grade for recombinant DNA products
- Superdex 200 prep grade separates MAbs from dimers and contaminants
- Available in prepacked HiLoad columns for quick method development
Technical Information
Superdex™ prep grade prepacked columns and lab packs.
| TECHNICAL SPECIFICATIONS |
| Superdex™ prep grade |
| Fractionation range (Mr) | |
| - Globular proteins | |
| Superdex™ 30 prep grade | < 1 × 104 |
| Superdex™ 75 prep grade | 3 × 103-7 × 104 |
| Superdex™ 200 prep grade | 1 × 104-6 × 105 |
| - Dextrans |
| Superdex™ 75 prep grade | 5 × 102-3 × 104 |
| Superdex™ 200 prep grade | 1 × 103-1 × 105 |
| Matrix | Spherical composite of cross-linked
agarose and dextran |
| Average particle size | 34 µm |
| Chemical stability | Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning-in-place) |
| pH stability | 3-12 (working and long term) |
| | 1-14 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Prepacked HiLoad™ Columns |
| Bed volume | HiLoad™ 16/60: 120 ml
HiLoad™ 26/60: 320 ml |
| Sample volume | HiLoad™ 16/60: < 5 ml
HiLoad™ 26/60: < 13 ml |
| Linear flow rate | 10-50 cm/h |
| Max. pressure | |
| Over the packed bed during operation: |
3 bar (42 psi, 0.3 MPa) |
| Column hardware: | 5 bar (73 psi, 0.5 MPa) |
| Theoretical plates | > 13 000 m-1 |
Simple and predictable five-fold scale-up of a mouse monoclonal IgG1 separation from Superdex™ 200 (13-µm particle size) to Superdex™ 200 prep grade (34-µm particle size).