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GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg
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产品名称:
GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg
产品型号:
GE Pharmacia 17-0673-01 蛋白肽寡聚核苷酸多糖半制备和分离纯化
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg ：蛋白，肽，寡聚核苷酸及多糖的半制备和高性能分析纯化。 球蛋白分离范围：10,000-600,000
GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg
的详细介绍
GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg
- Achieve high-resolution separations across exceptionally broad molecular-weight ranges.
- Easy, efficient scale-up from prepacked Superose™ 10/300 GL columns to Superose™ prep grade lab packs.
- Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose™ for some lipids, peptides, and small aromatic compounds.
- Tricorn™ high-performance column (10/300 GL) prepacked with Superose™ allows even distribution of eluent across the column bed to enable high-resolution separations.
- Precision columns (PC) for micropurification and analysis¾10-fold smaller column volume than Tricorn™ columns.
Technical Information
Superose™ 6 is designed for high-resolution chromatography. Superose™ 6 prep grade permits easy, efficient scale-up to preparative separations. Superose™ 12 is for high resolution purification of proteins from 1 × 103 to 3 × 105 molecular weight, while Superose™ 12 prep grade is useful for preparative purification of these higher molecular weight proteins.
| TECHNICAL SPECIFICATIONS |
| Superose™ 10/300 GL Columns (Tricorn™)* |
| Bed volume | 24 ml |
| Sample loading capacity | 5-10 mg, 240 µl |
| Recommended flow rate | |
| Superose™ 6 | 0.3-0.5 ml/min |
| Superose™ 12 | 0.5-1.0 ml/min |
| Max. pressure | |
| Superose™ 6 | 15 bar (217 psi, 1.5 MPa) |
| Superose™ 12 | 30 bar (435 psi, 3 MPa) |
| Particle size | |
| Superose™ 6 | 13 ± 2 µm |
| Superose™ 12 | 10 ± 2 µm |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Chemical stability | Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
1% SDS, organic solvents,
0.5 M NaOH (for cleaning-in-place) |
| pH stability | 3-12 (working and long term) |
| | 1-14 (short term) |
| Theoretical plates | |
| Superose™ 6 | > 30 000 m-1 |
| Superose™ 12 | > 40 000 m-1 |
| Superose PC 3.2/30 |
| Bed volume | 2.4 ml |
| Max. loading capacity | 200 µl |
| Max. flow rate | |
| Superose 6 | 0.1 ml/min |
| Superose 12 | 0.1 ml/min |
| Max. pressure | |
| Superose 6 | 12 Bar (1.2MPa / 175 psi) |
| Superose 12 | 24 Bar (2.4MPa / 350 psi) |
| Particle size | |
| Superose 6 | 13 ± 2 µm |
| Superose 12 | 10 ± 2 µm |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place) |
| pH stability | 3-12 (working and long term) 1-14 (short term) |
| Theoretical plates | |
| Superose 6 | > 30 000 m-1 |
| Superose 12 | > 40 000 m-1 |
| * Columns are not suitable for use with ÄKTAprime™ plus system. |
| TECHNICAL SPECIFICATIONS |
| Superose™ prep grade |
| Exclusion limit (Mr) | |
| Superose™ 6 prep grade | 4 × 107 protein |
| Superose™ 12 prep grade | 2 × 106 protein |
| Separation range (Mr) | |
| Superose™ 6 prep grade | 5 × 103-5 × 106 protein |
| Superose™ 12 prep grade | 1 × 103-3 × 105 protein |
| Matrix | Highly cross-linked agarose |
| Particle size | 30 ± 10 µm |
| Recommended linear flow rate | |
| Superose™ 6 prep grade | up to 40 cm/h |
| Superose™ 12 prep grade | up to 40 cm/h |
| Max. pressure | |
| Superose™ 6 prep grade: | 4 bar (58 psi, 0.4 MPa) |
| Superose™ 12 prep grade: | 7 bar (101 psi, 0.7 MPa) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Columns are not suitable for use with ÄKTAprime™ plus system. |
Separation of DNA fragments on Superose™ 6 HR 10/30 column (now available as Superose 6 10/300 GL column). Numbers above peaks correspond to the number of base pairs. Reproduced by kind permission of the authors and publisher.
GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg GE凝胶过滤预装住 HiLoad 16/60 Superdex 200 pg