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GE大分离范围凝胶过滤预装柱 Superose 6 10/300 GL
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产品名称:
GE大分离范围凝胶过滤预装柱 Superose 6 10/300 GL
产品型号:
GE Pharmacia 17-5172-01 蛋白的制备和纯化肽制备纯化
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
GE大分离范围凝胶过滤预装柱 Superose 6 10/300 GL ：蛋白的制备和纯化肽制备纯化， 球蛋白分离范围：5,000-5,000,000(D)
GE大分离范围凝胶过滤预装柱 Superose 6 10/300 GL
的详细介绍
GE大分离范围凝胶过滤预装柱 Superose 6 10/300 GL
Technical Information
Superose™ 6 is designed for high-resolution chromatography. Superose™ 6 prep grade permits easy, efficient scale-up to preparative separations. Superose™ 12 is for high resolution purification of proteins from 1 × 103 to 3 × 105 molecular weight, while Superose™ 12 prep grade is useful for preparative purification of these higher molecular weight proteins.
| TECHNICAL SPECIFICATIONS |
| Superose™ 10/300 GL Columns (Tricorn™)* |
| Bed volume | 24 ml |
| Sample loading capacity | 5-10 mg, 240 µl |
| Recommended flow rate | |
| Superose™ 6 | 0.3-0.5 ml/min |
| Superose™ 12 | 0.5-1.0 ml/min |
| Max. pressure | |
| Superose™ 6 | 15 bar (217 psi, 1.5 MPa) |
| Superose™ 12 | 30 bar (435 psi, 3 MPa) |
| Particle size | |
| Superose™ 6 | 13 ± 2 µm |
| Superose™ 12 | 10 ± 2 µm |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Chemical stability | Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
1% SDS, organic solvents,
0.5 M NaOH (for cleaning-in-place) |
| pH stability | 3-12 (working and long term) |
| | 1-14 (short term) |
| Theoretical plates | |
| Superose™ 6 | > 30 000 m-1 |
| Superose™ 12 | > 40 000 m-1 |
| Superose PC 3.2/30 |
| Bed volume | 2.4 ml |
| Max. loading capacity | 200 µl |
| Max. flow rate | |
| Superose 6 | 0.1 ml/min |
| Superose 12 | 0.1 ml/min |
| Max. pressure | |
| Superose 6 | 12 Bar (1.2MPa / 175 psi) |
| Superose 12 | 24 Bar (2.4MPa / 350 psi) |
| Particle size | |
| Superose 6 | 13 ± 2 µm |
| Superose 12 | 10 ± 2 µm |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place) |
| pH stability | 3-12 (working and long term) 1-14 (short term) |
| Theoretical plates | |
| Superose 6 | > 30 000 m-1 |
| Superose 12 | > 40 000 m-1 |
| * Columns are not suitable for use with ÄKTAprime™ plus system. |
| TECHNICAL SPECIFICATIONS |
| Superose™ prep grade |
| Exclusion limit (Mr) | |
| Superose™ 6 prep grade | 4 × 107 protein |
| Superose™ 12 prep grade | 2 × 106 protein |
| Separation range (Mr) | |
| Superose™ 6 prep grade | 5 × 103-5 × 106 protein |
| Superose™ 12 prep grade | 1 × 103-3 × 105 protein |
| Matrix | Highly cross-linked agarose |
| Particle size | 30 ± 10 µm |
| Recommended linear flow rate | |
| Superose™ 6 prep grade | up to 40 cm/h |
| Superose™ 12 prep grade | up to 40 cm/h |
| Max. pressure | |
| Superose™ 6 prep grade: | 4 bar (58 psi, 0.4 MPa) |
| Superose™ 12 prep grade: | 7 bar (101 psi, 0.7 MPa) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Columns are not suitable for use with ÄKTAprime™ plus system. |
GE大分离范围凝胶过滤预装柱 Superose 6 10/300 GLGE大分离范围凝胶过滤预装柱 Superose 6 10/300 GLGE大分离范围凝胶过滤预装柱 Superose 6 10/300 GL
Superose™ 6 is designed for high-resolution chromatography. Superose™ 6 prep grade permits easy, efficient scale-up to preparative separations. Superose™ 12 is for high resolution purification of proteins from 1 × 103 to 3 × 105 molecular weight, while Superose™ 12 prep grade is useful for preparative purification of these higher molecular weight proteins.