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GE凝胶过滤预装柱 HiPrep 16/60 Sephacryl S-400 HR
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产品名称:
GE凝胶过滤预装柱 HiPrep 16/60 Sephacryl S-400 HR
产品型号:
GE Pharmacia 28-9356-04 多糖的纯化大分子纯化
产品展商:
上海嘉适科学仪器有限公司
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简单介绍
GE凝胶过滤预装柱 HiPrep 16/60 Sephacryl S-400 HR ：多糖，具有大分子（如蛋白多糖，脂质体等小于25，000 bp DNA限制片段）。球蛋白分离范围：20,000-8,000,000
GE凝胶过滤预装柱 HiPrep 16/60 Sephacryl S-400 HR
的详细介绍
GE凝胶过滤预装柱 HiPrep 16/60 Sephacryl S-400 HR
HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs
Technical Information
HiPrep™ Sephacryl™ HR columns, prepacked with Sephacryl™ High Resolution media, provide reliable gel filtration separations.
HiPrep™ Sephacryl™ HR prepacked gel filtration columns offer excellent separation properties in a convenient, ready-to-use format. By combining the performance of Sephacryl™ High Resolution media with a low cost column construction, they are both reliable and cost effective to use with simple equipment or systems.
Five HiPrep™ Sephacryl™ HR columns are available prepacked with S-100, S-200, S-300, S-400, or S-500 HR media, respectively. Each column is also available in two sizes: 16/60 for sample volumes of up to 5 ml and 26/60 for sample volumes of up to 13 ml. The range of HiPrep™ Sephacryl™ HR prepacked columns allows purification of molecules with molecular weights as small as 1 × 103 to macromolecules as large as 2 × 107.
| TECHNICAL SPECIFICATIONS |
| Sephacryl™ High Resolution |
| Fractionation range (Mr) - | |
| - Globular proteins | |
S-100 HR
S-200 HR
S-300 HR
S-400 HR | 1 × 103 - 1 × 105
5 × 103 - 2.5 × 105
1 × 104 - 1.5 × 106
2 × 104 - 8 × 106 |
| - Dextrans | |
S-200 HR
S-300 HR
S-400 HR
S-500 HR
S-1000 HR | 1 × 103 - 8 × 104
2 × 103 - 4 × 105
1 × 104 - 2 × 106
4 × 104 - 2 × 107
5 × 105 - > 108 |
| Exclusion limit up to 400 nm for spherical particles. |
| - DNA exclusion limit | |
S-200 HR
S-300 HP
S-400 HR
S-500 HR
S-1000 HR | 30 bp
118 bp
271 bp
1078 bp
20 000 bp |
| Matrix | Spherical allyl dextran and N, N’-methylenebisacrylamide |
| Chemical stability | Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine-HCl, 1% SDS,
2 M NaCl, 24% ethanol,
30% propanol, 30% acetonitrile,
0.5 M NaOH (for cleaning) |
| pH stability | 3-11 (long term and working)
2-13 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
Physical stability
Autoclavability
Antimicrobial agent | Negligible volume variation due to changes in pH or ionic strength
At 121°C, pH 7 for 30 minutes (gel only)
20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
Selectivity of the five different prepacked HiPrep Sephacryl™ HR columns.
| TECHNICAL SPECIFICATIONS |
| Prepacked HiPrep™ Sephacryl HR Columns |
| Bed dimensions | |
HiPrep™ 16/60
HiPrep™ 26/60 | 16 × 600 mm
26 × 600 mm |
| Bed volume | |
HiPrep™ 16/60
HiPrep™ 26/60 | 120 ml
320 ml |
| Sample volume | |
HiPrep™ 16/60
HiPrep™ 26/60 | < 5 ml
< 13 ml |
| Rec. linear flow rate | 5-15 cm/h |
| Max. linear flow rate | 30 cm/h |
| (H2O at 25°C) | |
| Max. pressure over the packed bed during operation | 1.5 bar (22 psi, 0.15 MPa) |
| Column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) |
| Theoretical plates | 5000 m-1 |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
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