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GE预装脱盐柱 Disposable PD-10 Desalting Columns
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产品名称:
GE预装脱盐柱 Disposable PD-10 Desalting Columns
产品型号:
GE Pharmacia 17-0851-01 一次性脱盐缓冲液交换制备
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
GE预装脱盐柱 Disposable PD-10 Desalting Columns IMPROVED：一次性脱盐缓冲液交换制备。 球蛋白分离范围：1,000-5,000 柱体积：8.3ml
GE预装脱盐柱 Disposable PD-10 Desalting Columns
的详细介绍
GE预装脱盐柱 Disposable PD-10 Desalting Columns
Technical Information
PD-10 columns are packaged with a convenient column stand¾the PD-10 Desalting Workmate. Use LabMate Buffer Reservoir for easier equilibration.
Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.
The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol (order separately).
Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.
| TECHNICAL SPECIFICATIONS |
| Disposable PD-10 Desalting Columns |
| Matrix | Sephadex G-25 Medium |
| Separation mechanism | According to size |
| Particle size range | 85-260 µm |
| Exclusion limit | Mr 5000 |
| Sample volume (gravity) | 1.0 to 2.5 ml |
| Sample volume (spin) | 1.75 to 2.5 ml |
| Column volume | 13.5 ml |
| Medium volume | 8.3 ml |
| Column material | Polypropylene barrel and polyethylene frits |
| Chemical stability | All commonly used buffers |
| Working pH range | 2¾13 |
| Storage temperature | 4°C to 30°C |
Removal of NaCl from BSA using the gravity protocol. Recovery of the sample was 95% and the desalting capacity was > 99%.
PD-10 Desalting Columns give fast desalting with gravity flow or spin protocols. The blue adapters are used together with the spin protocols.
GE预装脱盐柱 Disposable PD-10 Desalting Columns