产品[
Phospho-4E-BP1 (Thr70) Antibody 磷酸- 4E - BP1 （Thr70）抗体
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产品名称:
Phospho-4E-BP1 (Thr70) Antibody 磷酸- 4E - BP1 （Thr70）抗体
产品型号:
cell signaling technology 9455
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
Phospho-4E-BP1 (Thr70) Antibody 磷酸- 4E - BP1 （Thr70）抗体 检测的4E - BP1 磷酸化苏氨酸只有在70内源性水平。这种抗体不会检测4E - BP1的磷酸化
Phospho-4E-BP1 (Thr70) Antibody 磷酸- 4E - BP1 （Thr70）抗体
的详细介绍
| No. | Size | Price |
|---|
| 9455L | 300 ul ( 30 western blots ) | |
| 9455S | 100 ul ( 10 western blots ) |
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|
| W IP | H M R Mk | Endogenous | 15 to 20 | Rabbit |
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
- 9455:
- Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Phospho-4E-BP1 (Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70. This antibody does not detect 4E-BP1 phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of rat 4E-BP1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
)
Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indicated number of minutes, using Phospho-4E-BP1 (Thr70) Antibody (upper) or 4E-BP1 Antibody #9452 (lower). (293 cells provided by Dr. Nahum Sonenberg, McGill University, Canada.)