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4E-BP1 (53H11) Rabbit mAb 4E - BP1（53H11）兔单克隆抗
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产品名称:
4E-BP1 (53H11) Rabbit mAb 4E - BP1（53H11）兔单克隆抗
产品型号:
cell signaling technology 9644
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
4E-BP1 (53H11) Rabbit mAb 4E - BP1（53H11）兔单克隆抗 检测总4E - BP1的内源性蛋白水平
4E-BP1 (53H11) Rabbit mAb 4E - BP1（53H11）兔单克隆抗
的详细介绍
| No. | Size | Price |
|---|
| 9644 | 100 ul ( 10 western blots ) |
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|
| W IP IHC-P IF-IC F | H M R Mk | Endogenous | 15-20 | Rabbit IgG |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
- 9644:
- Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
Specificity / Sensitivity
4E-BP1 (53H11) Rabbit mAb detects endogenous levels of total 4E-BP1 protein.
Source / Purification
4E-BP1 (53H11) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1.
Western Blotting
)
Western blot analysis of extracts from various cell lines using 4E-BP1 (53H11) Rabbit mAb.
IHC-P (paraffin)
)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 4E-BP1 (53H11) Rabbit mAb in the presence of control peptide (left) or 4E-BP1 blocking peptide #1053 (right).
IHC-P (paraffin)
)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using 4E-BP1 (53H11) Rabbit mAb.
IHC-P (paraffin)
)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using 4E-BP1 (53H11) Rabbit mAb.
IHC-P (paraffin)
)
Immunohistochemical analysis of paraffin-embedded mouse spleen, 4E-BP1/2 wild type (left) or 4E-BP1 knockout (right), using 4E-BP1 (53H11) Rabbit mAb. 4E-BP wild type and knockout tissues kindly provided by Dr. Nahum Sonenberg, McGill University.
Flow Cytometry
)
Flow cytometric analysis of COS cells using 4E-BP1 (53H11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
)
Confocal immunofluorescent analysis of HeLa cells using 4E-BP1 (53H11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).