产品[
4E-BP2 Antibody 4E-BP2抗体
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产品名称:
4E-BP2 Antibody 4E-BP2抗体
产品型号:
cell signaling technology 2845
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
4E-BP2 Antibody 4E-BP2抗体 检测总4E- BP2，独立的内源性磷酸化水平 这种抗体不交叉反应显着4E - BP1
4E-BP2 Antibody 4E-BP2抗体
的详细介绍
| No. | Size | Price |
|---|
| 2845 | 100 ul ( 10 western blots ) |
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|
| W IP IHC-P F | H M R Mk B | Endogenous | 15 to 20 | Rabbit |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine
Species cross-reactivity is determined by Western blot.
Protocols
- 2845:
- Flow, IHC / Paraffin, Immunoprecipitation, Western Blotting
Specificity / Sensitivity
4E-BP2 Antibody detects endogenous levels of total 4E-BP2, independent of phosphorylation. This antibody does not cross-react significantly with 4E-BP1.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy-terminus of human 4E-BP2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
)
Western blot analysis of extracts from A673 cells, untreated or nocodazole-treated (100 ng/ml, 16 hrs), using 4E-BP2 Antibody (upper) or 4E-BP1 Antibody #9452 (lower). Extracts were treated with lambda phosphatase NEB#P0753 (10,000 U/ml for 1 hour) to dephosphorylate both proteins.
Western Blotting
)
Western blot analysis of bacterially expressed GST-4E-BP1 and of extracts from NIH/3T3 cells, using 4E-BP2 Antibody and 4E-BP1 Antibody #9452.
IHC-P (paraffin)
)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic and nuclear localization, using 4E-BP2 Antibody.
IHC-P (paraffin)
)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using 4E-BP2 Antibody.
IHC-P (paraffin)
)
Immunohistochemical analysis of paraffin-embedded human follicular carcinoma (thyroid), using 4E-BP2 Antibody.
Flow Cytometry
)
Flow cytometric analysis of HeLa cells, using 4E-BP2 Antibody (blue) compared to a nonspecifc negative control antibody (red).
4E-BP2 Antibody 4E-BP2抗体 4E-BP2 Antibody 4E-BP2抗体 4E-BP2 Antibody 4E-BP2抗体