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GE 迷你型蛋白核酸垂直电泳槽 迷你型电泳仪
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产品名称:
GE 迷你型蛋白核酸垂直电泳槽 迷你型电泳仪
产品型号:
GE MiniVE 80-6418-77 垂直电泳仪
产品展商:
上海嘉适科学仪器有限公司
产品文档:
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简单介绍
GE 迷你型蛋白核酸垂直电泳槽 迷你型电泳仪最新设计的MiniVE为您带来无限惊喜！它采用聚丙烯酰胺凝胶分离生物大分子，如蛋白核酸等；进一步简化灌胶、跑胶和印迹步骤，却不失优质的实验结果。MiniVE功能全面、方便实用，是实验室常规蛋白电泳和转印操作的最佳选择！ 特点：灌胶跑胶同一模具，操作简便；缓冲液用量少，经济实用；功能强大，亦可用于2-D电泳；电泳模具兼容转印，一物多用。
GE 迷你型蛋白核酸垂直电泳槽 迷你型电泳仪
的详细介绍
技术参数 |
规格 | 18.7cm (h) * 20.3 cm (w) |
重量 | 1.15kg |
凝胶厚度 | 0.75-1.5 mm |
玻璃板尺寸 | 10 * 10.5 cm |
最少上槽缓冲液体积 | 60ml (10*8 cm ) 100ml (10*10.5 cm ) |
最少下槽缓冲液体积 | 700ml |
最少转印用缓冲液体积 | 300ml |
耐受温度极限 跑胶模具 转印模具 | 5-75℃ 5-75℃ |
耐受功率极限 跑胶模具 转印模具 | 25W 15W |
MiniVE功能全面、方便实用，是实验室常规蛋白电泳和转印操作的最佳选择！
·单元式设计、无琐碎零件，仅包括凝胶模具、印迹模具、下层缓冲液槽和安全电源盖；
·灌胶、跑胶同一模具，操作简便；
·缓冲液用量少，经济实用，即使同时进行4快凝胶转印也只需300ml缓冲液，最大程度地节约您的试剂消耗；
·功能强大，亦可用于2-D电泳；
·电泳模具兼容转印，无需额外购买转印装置，只需购买转印三明治夹和海绵即可进行Western blot；
·实现高通量灌胶；
·零配件可单独购买，维修成本低。
miniVE Vertical Electrophoresis System
Technical Information
Perform electrophoresis and electrotransfer using the compact miniVE Vertical Electrophoresis System, shown here with two gel modules and optional blot module.
miniVE System Blot Module can accept two gel/membrane stacks simultaneously.
Convenient Design
The simple, modular design of miniVE Vertical Electrophoresis System consolidates all the small parts for electrophoresis and blotting into one convenient unit. Uniform gel temperature is maintained with the tank-style lower buffer chamber. A full tank stabilizes the temperature so that lanes are straight and bands are flat. The Blot Module will accept two gel/membrane stacks simultaneously. The lower buffer chamber is designed to hold two Blot Modules, allowing four gels to be blotted at once.
Casting gels for miniVE System is as easy as load, ...
... clamp, ...
... and cast.
Load, Clamp, and Cast
The gel module is simultaneously a casting stand and an upper buffer chamber with integrated clamps. Simply assemble the gel sandwich of plates and spacers, clamp it onto the upper buffer chamber, then pivot the sealing gasket and cast the gel. After polymerization, simply release the bottom seal and drop the assembly into the tank for running. No extra parts are needed and no unclamping until the run is finished.
Two-fold serial dilutions of a crude E. coli protein extract and samples of purified recombinant green fluorescent protein were separated on a 12% polyacrylamide SDS gel run on miniVE. Gel was stained with Coomassie™ Blue.
Detection of E. coli GroEL following Western transfer in miniVE Blot Module. Two-fold serial dilutions of a crude protein extract were separated on a 12% polyacrylamide SDS gel run on miniVE. Proteins were transferred to Hybond™-P membrane using miniVE Blot Module. GroEL was detected using an anti-GroEL antibody, ECL Plus™ Western Blotting Detection System, and Hyperfilm™ ECL™.
Extra Length and Compatible Dimensions
The miniVE handles gel cassettes up to 10 × 10.5 cm (W × H), which increases the separation length by more than 30% over conventional 10 × 8 cm gels. In addition, miniVE is compatible with the glass plates, combs, and spacers of SE 260 Mini-Vertical units. For gradient gels, mini-Vertical Gel Casters can produce up to four identical gels at one time.
The Right Power Supplies
EPS 301 is the power supply of choice for the miniVE System. With ratings of 300 V and 400 mA, it is capable of both running and blotting gels. For faster transfers when blotting four gels at a time, use the EPS 2A200 for its higher current capability.
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