MonoBeads™ Columns

• Innovative, monodisperse 10-μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
• MonoBeads™ available in a choice of three prepacked column formats¾Tricorn™, HR, and PC columns.
• Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
• Compatible with ÄKTAdesign™, FPLC™ System, and other high-performance LC systems.
• MonoBeads™ are also available as Mono P for chromatofocusing (see Mono P™ Columns).

• Technical Information

  
  MonoBeads™ are prepacked in Tricorn™, HR, and PC column formats. The three Tricorn™ (5/50 GL, 10/100 GL, and 4.6/100 PE) columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads™ columns (see page 511).

   

  Tricorn™ columns are ideal for the polishing step when high purity is required. HR 16/10 columns, on the other hand, have higher loading capacity than Tricorn™ columns. PC 1.6/5 columns are ideal for micropurification of subnanogram to microgram amounts of sample on ÄKTA Systems or other high-performance LC systems.

   

  Customer feedback on Mono Q HR 5/5.

   

  TECHNICAL SPECIFICATIONS
  MonoBeads™
  Ion exchanger type	Mono Q™	Strong anion
  	Mono S™	Strong cation
  Ionic capacity	Mono Q™	0.27-0.37 mmol Cl-/ml
  	Mono S™	0.12-0.15 mmol H+/ml
  pH stability		2-12 (working), 1-14 (short term)
  Chemical stability		Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid.
  Medium		Monodisperse polystyrene/divinyl benzene particles
  Particle size		10 μm
  Storage		20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S)
  Storage temperature		4°C to 30°C
  MonoBeads Columns*
  Bed volume	PC 1.6/5	0.1 ml
  	5/50 GL	1 ml
  	4.6/100 PE	1.7 ml
  	10/100 GL	8 ml
  	HR 16/10	20 ml
  Max. pressure	PC 1.6/5	50 bar (725 psi, 5 MPa)
  	5/50 GL, 10/100 GL,  4.6/100 PE	40 bar (580 psi, 4 MPa)
  	HR 16/10	30 bar (435 psi, 3 MPa)
  Recommended flow rate	PC 1.6/5	0.01-0.4 ml/min
  	5/50 GL, 4.6/100 PE	0.5-3.0 ml/min
  	10/100 GL	2.0-6.0 ml/min
  	HR 16/10	< 10 ml/min
  * Columns are not suitable for use with ÄKTAprime™ plus system.

   

  
  Tricorn high-performance columns are prepacked with MonoBeads ion exchange media for high-resolution purification and high capacity.

   

  
  The polishing step on Mono Q 5/50 GL shows different phosphoforms of the purified kinase and very high purity. GE Mono Q 10/100 GL MonoBeads™ Columns MonoQ预装柱