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GE Mono S 10/100 GL MonoBeads™ Columns MonoQ预装柱
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产品名称:
GE Mono S 10/100 GL MonoBeads™ Columns MonoQ预装柱
产品型号:
GE 17-5169-01 半制备 高分辨率 蛋白纯化分析
产品展商:
上海嘉适科学仪器有限公司
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简单介绍
GE Mono S 10/100 GL MonoBeads™ Columns MonoQ预装柱应用：蛋白纯化和分析； 每毫升载量：75mg IgG
GE Mono S 10/100 GL MonoBeads™ Columns MonoQ预装柱
的详细介绍
MonoBeads™ Columns
- Innovative, monodisperse 10-μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
- MonoBeads™ available in a choice of three prepacked column formats¾Tricorn™, HR, and PC columns.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- Compatible with ÄKTAdesign™, FPLC™ System, and other high-performance LC systems.
- MonoBeads™ are also available as Mono P for chromatofocusing (see Mono P™ Columns).
MonoBeads™ Columns
Technical Information
MonoBeads™ are prepacked in Tricorn™, HR, and PC column formats. The three Tricorn™ (5/50 GL, 10/100 GL, and 4.6/100 PE) columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads™ columns (see page 511).
Tricorn™ columns are ideal for the polishing step when high purity is required. HR 16/10 columns, on the other hand, have higher loading capacity than Tricorn™ columns. PC 1.6/5 columns are ideal for micropurification of subnanogram to microgram amounts of sample on ÄKTA Systems or other high-performance LC systems.
Customer feedback on Mono Q HR 5/5.
| TECHNICAL SPECIFICATIONS |
| MonoBeads™ |
| Ion exchanger type | Mono Q™ | Strong anion |
| | Mono S™ | Strong cation |
| Ionic capacity | Mono Q™ | 0.27-0.37 mmol Cl-/ml |
| | Mono S™ | 0.12-0.15 mmol H+/ml |
| pH stability | | 2-12 (working), 1-14 (short term) |
| Chemical stability | | Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid. |
| Medium | | Monodisperse polystyrene/divinyl benzene particles |
| Particle size | | 10 μm |
| Storage | | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) |
| Storage temperature | 4°C to 30°C |
| MonoBeads Columns* |
| Bed volume | PC 1.6/5 | 0.1 ml |
| | 5/50 GL | 1 ml |
| | 4.6/100 PE | 1.7 ml |
| | 10/100 GL | 8 ml |
| | HR 16/10 | 20 ml |
| Max. pressure | PC 1.6/5 | 50 bar (725 psi, 5 MPa) |
| | 5/50 GL, 10/100 GL,
4.6/100 PE | 40 bar (580 psi, 4 MPa) |
| | HR 16/10 | 30 bar (435 psi, 3 MPa) |
| Recommended flow rate | PC 1.6/5 | 0.01-0.4 ml/min |
| | 5/50 GL, 4.6/100 PE | 0.5-3.0 ml/min |
| | 10/100 GL | 2.0-6.0 ml/min |
| | HR 16/10 | < 10 ml/min |
* Columns are not suitable for use with ÄKTAprime™ plus system.
|
Tricorn high-performance columns are prepacked with MonoBeads ion exchange media for high-resolution purification and high capacity.
The polishing step on Mono Q 5/50 GL shows different phosphoforms of the purified kinase and very high purity.
GE Mono S 10/100 GL MonoBeads™ Columns MonoQ预装柱GE Mono S 10/100 GL MonoBeads™ Columns MonoQ预装柱GE Mono S 10/100 GL MonoBeads™ Columns MonoQ预装柱GE离子交换预装柱价格参数GE 17-5169-01 半制备 高分辨率 蛋白纯化分析GE 17-5169-01 半制备 高分辨率 蛋白纯化分析