His MultiTrap 96-Well Filter Plates - High-throughput Screening

• Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* proteins.
• Increased convenience and consistency with prepacked 96-well plates.
• No filtration needed¾load unclarified sample directly, increase reproducibility, and save time.
• Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
• Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns.
• High chemical stability and high binding capacity¾up to 1 mg of histidine-tagged protein per well.
  
  His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose™ 6 Fast Flow (His MultiTrap™ FF) or Ni Sepharose™ High Performance (His MultiTrap™ HP). A protocol for His MultiTrap is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.

   

  Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target proteins. His MultiTrap™ FF and His MultiTrap HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.

   

  
  His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for expression screening and small-scale purification of histidine-tagged proteins.

   

  
  SDS-PAGE of (His)₆-NURR1 purified with 96 different buffer conditions on His MultiTrap™ FF. Arrows indicate the position of the target protein. The results show that the highest amount of purified protein was recovered in the buffer at pH 8.5 (25 mM Tris, 100 mM NaCl and 10% glycerol, pH 8.5). This work was performed by R. Steele and B. L. Grasberger at Johnson & Johnson Pharmaceutical Research & Development LCC, USA. Published with permission of the authors.

   

  For the full range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.

   

  
  

  His MultiTrap 96-Well Filter Plates - High-throughput Screening

  Technical Information

  
  

  TECHNICAL SPECIFICATIONS
  Multiwell format	96 well
  Well volume	800 μl
  Medium volume	50 μl
  His MultiTrap FF His MultiTrap HP	Ni Sepharose 6 Fast Flow Ni Sepharose High Performance
  Max. sample loading volume	600 μl
  Binding capacity*
  His MultiTrap FF His MultiTrap HP	Up to 800 μg histidine-tagged protein/well Up to 1 mg histidine-tagged protein/well
  Reproducibility between wells	± 10% in yield
  pH stability†	2-14 (short term), 3-12 (long term)
  Storage	20% ethanol
  Storage temperature	4°C to 30°C
  * Binding capacity is protein-to-protein dependent. † Ni2+-stripped medium.

  
  His MultiTrap HP 96-Well Filter Plates - High-throughput ScreeningHis MultiTrap HP 96-Well Filter Plates - High-throughput Screening